Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus
نویسندگان
چکیده
Aqueous extracts and organic solvent extracts of isolated marine cyanobacteria strains were tested for antimicrobial activity against a fungus, Gram-positive and Gram-negative bacteria and for cytotoxic activity against primary rat hepatocytes and HL-60 cells. Antimicrobial activity was based on the agar diffusion assay. Cytotoxic activity was measured by apoptotic cell death scored by cell surface evaluation and nuclear morphology. A high percentage of apoptotic cells were observed for HL-60 cells when treated with cyanobacterial organic extracts. Slight apoptotic effects were observed in primary rat hepatocytes when exposed to aqueous cyanobacterial extracts. Nine cyanobacteria strains were found to have antibiotic activity against two Gram-positive bacteria, Clavibacter michiganensis subsp. insidiosum and Cellulomonas uda. No inhibitory effects were found against the fungus Candida albicans and Gram-negative bacteria. Marine Synechocystis and Synechococcus extracts induce apoptosis in eukaryotic cells and cause inhibition of Gram-positive bacteria. The different activity in different extracts suggests different compounds with different polarities.
منابع مشابه
Toxicity assessment of crude and partially purified extracts of marine Synechocystis and Synechococcus cyanobacterial strains in marine invertebrates.
Among the Cyanoprokaryota, the genera Synechocystis and Synechococcus have rarely been studied with respect to potential toxicity. This is particularly true with marine environments where studies about the toxicity of cyanobacteria are restricted to filamentous forms at the warmer temperate and tropical regions and also to filamentous forms at cold seas such as the Baltic Sea. In this study, we...
متن کاملIdentification and enumeration of marine chroococcoid cyanobacteria by immunofluorescence.
We used an indirect immunofluorescence technique to permit the identification and enumeration of individual or closely related strains of chroococcoid cyanobacteria of the general Synechococcus and Synechocystis in natural seawater samples. Antisera directed against each of five strains (two phycoerythrin-containing Synechococcus strains, two phycocyanin-containing Synechococcus strains, and on...
متن کاملمطالعه خواص ضدباکتریایی عصاره های متانولی، اتری و آبی برخی از گونه های سیانوباکتری ها در شرایط آزمایشگاهی
Background and purpose: Cyanobacteria are rich sources of secondary metabolites. Antibiotic resistant pathogens are rising and people are more interested in using natural products these days. Hence, identifying competent cyanobacteria for the extraction of antimicrobial compounds is of great benefit. The main objective of this study was to investigate the in vitro antibacterial activity of ...
متن کاملAntimicrobial activity of Cyanobacteria isolated from hot spring of Geno
Cyanobacteria, the blue green algae are one of the most diverse groups of Gram-negative photosynthetic prokaryotes widely distributed throughout the world. Cyanobacteria are known to produce a wide range of secondary metabolites with various biological actions. The aim of this study was to test extracts from various cyanobacteria isolated from Geno hot spring, Bandar Abbas (Iran) against a dive...
متن کاملDifferent strategies of metabolic regulation in cyanobacteria: from transcriptional to biochemical control
Cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 show similar changes in the metabolic response to changed CO2 conditions but exhibit significant differences at the transcriptomic level. This study employs a systems biology approach to investigate the difference in metabolic regulation of Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803. Presented multi-level kin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Marine Drugs
دوره 6 شماره
صفحات -
تاریخ انتشار 2008